{"id":274177,"date":"2026-06-23T11:38:35","date_gmt":"2026-06-23T10:38:35","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/?post_type=vf_event&#038;p=274177"},"modified":"2026-06-29T13:39:41","modified_gmt":"2026-06-29T12:39:41","slug":"fis27-01","status":"publish","type":"vf_event","link":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/fis27-01\/","title":{"rendered":"FISHing for RNAs: classical to single molecule approaches"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-overview\" data-vf-js-location-nearest-activation-target=\"\">Overview<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-sponsors\" data-vf-js-location-nearest-activation-target=\"\">Sponsors<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-mediakit\" data-vf-js-location-nearest-activation-target=\"\">Media kit<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-overview\"><h2 class=\"vf-u-sr-only\">Overview<\/h2>\n<p>Applications for this event are not open yet. If you are interested in receiving more information please register your interest.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Course overview<\/h2>\n\n\n\n<p>The development of transcriptome-wide RNA localization screens, together with the more recent advent of scRNA and spatial sequencing approaches, have revealed that RNA expression, processing and distribution are tightly regulated in space and time across species and cell types. These discoveries, performed in a range of biological systems (from cultured cells to organoids and model organisms), underscore the need to detect transcripts with both cellular and subcellular resolution and to quantitatively analyze their abundance and localization.<\/p>\n\n\n\n<p>This course will provide comprehensive theoretical and practical training covering all key steps and the latest developments in quantitative FISH methods, while also offering a broad perspective on their applications to a wide range of biological questions.<\/p>\n\n\n\n<p>Scientific talks by leaders in the field will additionally highlight application of ISH techniques in diverse biological models and introduce complementary methods to study RNA dynamics (e.g. localization, localized translation), along with transcriptome-wide and super-resolution approaches.<\/p>\n\n\n\n<p>Through dedicated networking activities, project presentations and interactive discussion sessions, participants will engage with a vibrant community of biologists working on spatially resolved RNA imaging.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Learning outcomes<\/h2>\n\n\n\n<p>In this course, participants will learn how to design, perform and quantitatively analyze in situ hybridization (ISH) experiments to detect specific RNA molecules and study their processing in a variety of biological samples. They will receive hands-on training in complementary methods, including single-molecule FISH and hybridization chain reaction. <\/p>\n\n\n\n<p>Practical modules will teach participants how to design robust, specific probes for diverse applications, how to fluorescently label them and how to use them for reliable and reproducible detection. Dedicated imaging and image analysis sessions will provide a solid foundation in best practices as well as knowledge on community-standard softwares.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">What past participants say about the course<\/h2>\n\n\n\n<p>&#8220;<em>The course &#8220;FISHing for RNAs: classical to single molecule approaches&#8221; was trulyoutstanding, providing not only in-depth theoretical and hands-on training, but also critical skillsin troubleshooting and problem-solving with these techniques. The networking was surprisinglyproductive, allowing me to build meaningful connections with both peers and organisers.<\/em>&#8221; &#8211; Carlotta Nardi, Universit\u00e0 degli Studi di Milano, Italy<\/p>\n\n\n\n<p>&#8220;<em>This EMBO Practical Course had a direct impact on my project by providing expert guidanceand hands-on training. The Keynote lectures further expanded my perspective and inspirednew research questions for my work.<\/em>&#8221; &#8211; Carolina Oliveira Rizzo, Universit\u00e4t Bern, Switzerland<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-sponsors\"><h2>Sponsors<\/h2>\n<p><\/p>\n\n\n\n<p><strong>Sponsorship opportunities<\/strong><\/p>\n\n\n\n<p>We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget.&nbsp;Discounts are available for companies sponsoring multiple events at EMBL Heidelberg.&nbsp;<a href=\"http:\/\/www.embl.de\/training\/events\/index.php?p_eventType=CON&amp;p_outstation=HD&amp;p_timeRange=FUTURE&amp;submit=Show+Events\">View other conferences<\/a>, or contact&nbsp;<a href=\"mailto:sponsorship@embl.de\">sponsorship@embl.org.<\/a>&nbsp;for further information.<\/p>\n\n\n\n<p>If you are interested in becoming a media partner of this event, please visit our&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/sponsor-an-event\/#vf-tabs__section-08208670-4ad4-45ca-a4ff-ac6edb6ffd0b\" target=\"_blank\" rel=\"noreferrer noopener\">media partnerships webpage<\/a>.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><p><a href=\"mailto:sponsorship@embl-em.de?subject=%20Request%20a%20Sponsorship%20Brochure&#038;body=Please%20kindly%20send%20me%20the%20sponsorship%20brochure%20for%20(please%20indicate%20the%20event%20your%20are%20interested%20in).\" class=\"vf-button vf-button--primary vf-button--pill\">Request a sponsorship brochure<\/a><\/p><\/div>\n\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quaternary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Warning                  <\/h3> \n        <p class=\"vf-box__text\">EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name \u2018Judy Eastman\u2019 (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL\u2019s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.<\/p>\n<p class=\"vf-box__text\">Please also note that:<\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">EMBL will never call or email you to ask for your credit card details or to request a payment.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">All payments are on invoice.<\/span><\/li>\n<\/ul>\n<p class=\"vf-box__text\">Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsoring@embl.de.<\/p>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-mediakit\"><h2>Media kit<\/h2>\n<p>Want to let others know you&#8217;re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.<\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"parent":0,"menu_order":0,"template":"","embl_taxonomy":[],"class_list":["post-274177","vf_event","type-vf_event","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"vf_event_start_date":{"value":"20270926","formatted":"26 September 2027"},"vf_event_end_date":{"value":"20271001","formatted":"1 October 2027"},"vf_event_submission_opening":{"value":"","formatted":""},"vf_event_submission_closing":{"value":"","formatted":""},"vf_event_registration_opening":{"value":"","formatted":""},"vf_event_registration_closing":{"value":"","formatted":""},"vf_event_summary":{"value":"","formatted":""},"vf_event_additional_info":{"value":"","formatted":""},"vf_event_registration_fee":{"value":"","formatted":""},"vf_event_canceled":{"value":"no","formatted":"no"},"vf_event_venue":{"value":"EMBL Advanced Training 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