{"id":17267,"date":"2021-10-28T08:09:16","date_gmt":"2021-10-28T07:09:16","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/?post_type=vf_event&#038;p=17267"},"modified":"2022-04-06T08:33:21","modified_gmt":"2022-04-06T07:33:21","slug":"scr22-02","status":"publish","type":"vf_event","link":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/scr22-02\/","title":{"rendered":"Gene expression at single-cell resolution &#8211; Cancelled"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-53de8397-0f08-449c-b4a4-0fdca47249f2\" data-vf-js-location-nearest-activation-target=\"\">Overview<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-53de8397-0f08-449c-b4a4-0fdca47249f2\"><h2 class=\"vf-u-sr-only\">Overview<\/h2>\n<p><\/p>\n\n\n\n<p>We are sorry to inform you that we have to cancel this course, and apologise for the inconvenience.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Course overview<\/h2>\n\n\n\n<p>The goal of this course is to provide participants with both the theory and practical skills required to design, prepare, run and analyze high-throughput single cell transcriptomic experiments using the Chromium Single Cell Controller from 10x Genomics. We will begin with a general introduction on the topic, including the cell partitioning technology, fundamentals of NGS, and the library preparation workflow. The hands-on session starts with cell handling followed by the preparation of transcriptome libraries from single-cells with a focus on best practices. Finally, we will quantify and quality control the prepared libraries, before sequencing on a NextSeq instrument. The final day will be dedicated to single-cell data analysis with a mixture of theory and hands-on experience using the Loupe Cell Browser from 10x Genomics.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Audience<\/h2>\n\n\n\n<p>This course is directed towards biologists, scientists and technicians who want to apply single-cell transcriptome sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology and cell culture is desirable but not essential.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Modules\/Resources<\/h2>\n\n\n\n<ul class=\"wp-block-list\"><li>Preparation of high-quality single-cell suspensions, flow cytometry<\/li><li>Best practices and \u201ctips and tricks\u201d for preparation of 3\u2019-tagged RNA-Seq libraries from single cells for whole transcriptome gene expression analysis<\/li><li>Best practices of sequencing library validation<\/li><li>Introduction to single cell transcriptome data analysis<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Learning outcomes<\/h2>\n\n\n\n<p>After this course you should be able to:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Prepare libraries from cell cultures for single-cell whole transcriptome analysis<\/li><li>Apply quality controls and library quantification<\/li><li>Know the basics of single-cell transcriptome data analysis<\/li><\/ul>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n<\/section>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"parent":0,"menu_order":0,"template":"","embl_taxonomy":[],"class_list":["post-17267","vf_event","type-vf_event","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"vf_event_start_date":{"value":"20220725","formatted":"25 July 2022"},"vf_event_end_date":{"value":"20220729","formatted":"29 July 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