{"id":171283,"date":"2024-07-04T13:52:51","date_gmt":"2024-07-04T12:52:51","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/?post_type=vf_event&#038;p=171283"},"modified":"2025-07-16T12:52:09","modified_gmt":"2025-07-16T11:52:09","slug":"neb25-01","status":"publish","type":"vf_event","link":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/neb25-01\/","title":{"rendered":"CANCELLED: NicE-RNA sequencing for multiomics on open chromatin and transcription"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-overview\" data-vf-js-location-nearest-activation-target=\"\">Overview<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-speakers\" data-vf-js-location-nearest-activation-target=\"\">Speakers<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-programme\" data-vf-js-location-nearest-activation-target=\"\">Preliminary programme<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-sponsors\" data-vf-js-location-nearest-activation-target=\"\">Sponsors<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-mediakit\" data-vf-js-location-nearest-activation-target=\"\">Media kit<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-overview\"><h2 class=\"vf-u-sr-only\">Overview<\/h2>\n<h2 class=\"wp-block-heading\">Update on cancellation<\/h2>\n\n\n\n<p>Thank you for your interest in the EMBL Course on NicE-RNA sequencing for multiomics on open chromatin and transcription.\u00a0<\/p>\n\n\n\n<p>Unfortunately, due to product release delays, we are not able to host this course in September 2025 and have to cancel the event.<\/p>\n\n\n\n<p>If you would like to receive further details on the product\/protocol please feel free to contact the NEB Senior Application Manager Dr. Bj\u00f6rn Textor.<\/p>\n\n\n\n<p>To stay in contact with us you can visit\u00a0<a href=\"http:\/\/www.embl.org\/events\" target=\"_blank\" rel=\"noreferrer noopener\">www.embl.org\/events\u200b<\/a>\u00a0for a complete list of all EMBL events and\u00a0subscribe for our newsletter at\u00a0<a href=\"http:\/\/www.embl.de\/events\/newsletter\" target=\"_blank\" rel=\"noreferrer noopener\">www.embl.de\/events\/newsletter\u200b<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Course overview<\/h2>\n\n\n\n<p>This course will focus on the latest state-of-the-art novel library preparation protocols. We will start with experimental design, focusing on hands-on library preparation (including best practices, standards, and potential pitfalls), concluding with sequencing of libraries, bioinformatic evaluation &amp; best practices.<\/p>\n\n\n\n<p>Multiomic data in scientific projects have become more and more important. NicE-Seq is a novel method enabling detection of open chromatin in fixed low numbers of cells without any additional instrumentation. Combining open chromatin with transcriptome information is crucial for interpretation of cellular states. Therefore, the course participants will also prepare mRNA libraries from the same cell populations.&nbsp;<\/p>\n\n\n\n<p>Participants will carry out a complete workflow, from isolation of DNA &amp; RNA from fixed cells, library preparation for both applications and shallow sequencing. The course will discuss alternative novel approaches, various cell fixatives and their impact on analysis. In addition, we will cover basic bioinformatic tools required for data processing, quality control and initial data analysis.<\/p>\n\n\n\n<p>Beside the practical exercises, lectures and discussion sessions, the course will offer the opportunity to learn more about transferring the knowledge gained during the course to the participants\u2019 home institutes.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Audience<\/h2>\n\n\n\n<p>This course is directed towards wet-lab biologists, scientists and technicians who want to apply the here offered technology.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Modules\/resources<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hands-on training on NGS multiome library preparations with upcoming state-of-the-art reagents<\/li>\n\n\n\n<li>Setting up a sequencing run<\/li>\n\n\n\n<li>Basics in data handling and analysis<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Learning outcomes<\/h2>\n\n\n\n<p>Multiome genomic analyses have become important to understand genome dynamics. Methodology to explore this is still challenging and requires a lot of experience and hands on time.<\/p>\n\n\n\n<p>During the course, its participants will:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>learn to apply novel NEBNext NGS library preparation reagents and protocols<\/li>\n\n\n\n<li>learn about the benefits and bottlenecks of different library preparations<\/li>\n\n\n\n<li>be exposed to cutting-edge sequencing technology<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">What past participants say about this course<\/h2>\n\n\n\n<p><em>\u201cI really enjoyed this course (both practical and invited lectures). This was an interesting course format and I will apply the techniques I learned during the course in my project. This is an extremely valuable course and I recommend this course to my colleagues. Thanks to the EMBL organizers for making such a wonderful event successful.\u201d&nbsp;\u2013<\/em>&nbsp;Kesava Priyan Parmasamy, Ulmea University, Sweden<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-speakers\"><h2 class=\"vf-u-sr-only\">Speakers<\/h2>\n<h2 class=\"wp-block-heading\">Speakers, trainers, and staff<\/h2>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Benes_Vladimir1-5.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Vladimir Benes            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/CP-60035060.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Mathias Boulanger            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Alicia He            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/CP-60024830.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Ferris Jung            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Keerthana Krishnan            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Chaithanya Ponnaluri            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Jan-Provaznik-1.jpg\" class=\"vf-profile__image\" alt=\"Jan_Provaznik\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Jan Provaznik            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Prummel.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Karin Prummel            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Chen Song            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-300x300.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Bj\u00f6rn Textor            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Laura-Villacorta.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Laura Villacorta            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Welter_Daphne.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Daphne Welter            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Scientific organisers<\/h2>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Benes_Vladimir1-5.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/people\/person\/benes\/\" target=\"_self\" class=\"vf-profile__link\">Vladimir Benes<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-300x300.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Textor_Bjorn.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/international.neb.com\/\" target=\"_self\" class=\"vf-profile__link\">Bj\u00f6rn Textor<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                New England Biolabs            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Laura-Villacorta.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/groups\/genomics\/\" target=\"_self\" class=\"vf-profile__link\">Laura Villacorta<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Course organisers<\/h2>\n\n\n\n<div class=\"embl-grid\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/CP-60044315.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Ellen Malovrh            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Scientific Training            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Officer            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/sites\/default\/files\/persons\/CP-60100455.jpg\" >  \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Ad\u00e9la Graf            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Course and Conference Officer            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"266\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-266x300.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-266x300.jpg 266w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-907x1024.jpg 907w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-768x867.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-1360x1536.jpg 1360w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Karoline_Wolkersdorfer-1814x2048.jpg 1814w\" sizes=\"auto, (max-width: 266px) 100vw, 266px\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Karo Wolkersdorfer            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Course and Conference            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Officer            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-programme\"><h2>Preliminary programme<\/h2>\n<p id=\"block-1984fb46-4b9e-4350-bb7b-3decc4627750\">Are you on social media? Post using<strong> #EMBLNicESeq <\/strong>and don&#8217;t forget to tag <strong>@EMBLEvents<\/strong>.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The programme will be shared here in due time.<\/li>\n\n\n\n<li>Some pre-course work (such as watching pre-recordings and protocol reading) with estimated time 0-10 hours will be required and will be shared in advance of the course.<\/li>\n\n\n\n<li>Some speakers may need to join virtually to give their lecture or Q&amp;A, and the programme is subject to change.<\/li>\n<\/ul>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 1 &#8211; Monday, 29 September 2025<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Description<\/th><\/tr><\/thead><tbody><tr><td>Arrival at EMBL + registration<\/td><\/tr><tr><td>Welcome<\/td><\/tr><tr><td><strong>EMBL intro and house notes (incl. safety) + course overview<br><\/strong>Vladimir Benes &#8211; EMBL Heidelberg, Germany<br>Bjoern Textor &#8211; New England Biolabs, USA<br>Laura Villacorta &#8211; EMBL Heidelberg, Germany<\/td><\/tr><tr><td><strong>Icebreaking activity<\/strong><br>Introduction round and \u201cChallenges and Expectations\u201d<\/td><\/tr><tr><td>Coffee break<\/td><\/tr><tr><td><strong>Lecture: <\/strong><strong>Sequencing technology overview and how to work in a Core facility<\/strong><br>Vladimir Benes<\/td><\/tr><tr><td><strong>Practical session: <\/strong><br>Cell fixation and preparation of DNA and RNA<\/td><\/tr><tr><td><strong>Tips &amp; Tricks <\/strong>with concurrent dinner<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 2 &#8211; Tuesday, 30 September 2025<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Description<\/th><\/tr><\/thead><tbody><tr><td><strong>Introduction Lecture: NicE-seq: High Resolution Profiling of Accessible Chromatin of Formaldehyde-fixed Cells<\/strong><br>Chaithanya Ponnaluri <\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Cytosol Extraction<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>NicE-Seq labeling reaction<\/td><\/tr><tr><td><strong>Lecture: Methods for sequencing cell-free DNA and cell-free RNA from human plasma<\/strong><br>Chen Song<\/td><\/tr><tr><td>Lunch<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Decrosslinking<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Clean-Up<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>EndPrep, Ligation, Buffer Exchange<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>PCR Reaction<\/td><\/tr><tr><td><strong>Lecture: Streamlining processes of automated RNA library prep<\/strong><br>Ferris Jung <\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Clean Up<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Library QC, Quantification<\/td><\/tr><tr><td>Dinner<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 3 &#8211; Wednesday, 1 October 2025<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Description<\/th><\/tr><\/thead><tbody><tr><td><strong>Lecture: Introduction into strand-specific RNA Library Prep: NicE-Seq application in the field \u2013 first hands-on experiences<\/strong><br>Ulrike B\u00f6nisch<\/td><\/tr><tr><td><strong>Practical Session:<\/strong> PolyA Enrichment<\/td><\/tr><tr><td><strong>Practical Session: <\/strong><br>Fragmentation<br>First Strand Synthesis<br>Second Strand Synthesis<\/td><\/tr><tr><td>Lunch<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Clean Up<\/td><\/tr><tr><td><strong>Practical Session:<\/strong> End prep of cDNA libraries<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Adaptor Ligation<br>USER Treatment<\/td><\/tr><tr><td><strong>Practical Session:<\/strong> PCR Enrichment<\/td><\/tr><tr><td><strong>Lecture: NEBNext Product Development: Streamlining workflows &amp; addressing new applications<\/strong><br>Keerthana Krishnan<\/td><\/tr><tr><td>Coffee Break<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Clean Up<\/td><\/tr><tr><td><strong>Practical Session: <\/strong>Library QC (Qubit, Bioanalyzer)<\/td><\/tr><tr><td>Dinner<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 4 &#8211; Thursday,  2 October 2025<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Time<\/th><th>Description<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>09:00 &#8211; 10:00<\/td><td><strong>Introduction &amp; Practical Session:<\/strong> <br>Set-up of Sequencing runs of processed samples<\/td><td>Training Labs<\/td><\/tr><tr><td>10:00 &#8211; 10:45<\/td><td><strong>Lecture: Epigenomic research <\/strong><br>Karin Prummel<\/td><td>Flex Labs<\/td><\/tr><tr><td>10:45 &#8211; 11:00<\/td><td>Coffee Break<\/td><td>Courtyard Foyer<\/td><\/tr><tr><td>11:00 &#8211; 12:00<\/td><td><strong>Practical Session: Bioinformatics I<br><\/strong>Introduction to data analysis and QC<\/td><td><\/td><\/tr><tr><td>12:00 &#8211; 13:00<\/td><td>Lunch<\/td><td>EMBL Canteen<\/td><\/tr><tr><td>13:00 &#8211; 14:30<\/td><td><strong>Practical Session: Bioinformatics II<br><\/strong>NicE-seq examples and pitfalls<\/td><td><\/td><\/tr><tr><td>14:30 &#8211; 16:00<\/td><td>Final technical discussion round<\/td><td>Flex Labs<\/td><\/tr><tr><td>16:00 &#8211; 16:45<\/td><td>Feedback round and closing remarks<\/td><td>Flex Labs<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-sponsors\"><h2>Sponsors<\/h2>\n<p><strong>Sponsorship opportunities<\/strong><\/p>\n\n\n\n<p>We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget.&nbsp;Discounts are available for companies sponsoring multiple events at EMBL Heidelberg.&nbsp;<a href=\"https:\/\/www.embl.org\/events\/\">View other events<\/a>, or contact&nbsp;<a href=\"mailto:sponsorship@embl.de\">sponsorship@embl.de<\/a>&nbsp;for further information.<\/p>\n\n\n\n<p>If you are interested in becoming a media partner of this event, please visit our&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/sponsor-an-event\/#vf-tabs__section-08208670-4ad4-45ca-a4ff-ac6edb6ffd0b\" target=\"_blank\" rel=\"noreferrer noopener\">media partnerships webpage<\/a>.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><p><a href=\"mailto:sponsorship@embl-em.de?subject=%20Request%20for%20a%20Sponsorship%20Brochure&#038;body=Please%20kindly%20send%20me%20the%20sponsorship%20brochure%20for%20EMBL%20Course:%20NicE-RNA%20sequencing%20for%20multiomics%20on%20open%20chromatin%20and%20transcription%20(29%20September%20-%202%20October%202025).\" class=\"vf-button vf-button--primary vf-button--pill\">Request a sponsorship brochure<\/a><\/p><\/div>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quaternary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Warning                  <\/h3> \n        <p class=\"vf-box__text\">EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name \u2018Judy Eastman\u2019 (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL\u2019s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.<\/p>\n<p class=\"vf-box__text\">Please also note that:<\/p>\n<ul>\n<li><span style=\"font-size: 12pt;\">EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.<\/span><\/li>\n<li><span style=\"font-size: 12pt;\">EMBL will never call or email you to ask for your credit card details or to request a payment.<\/span><\/li>\n<li><span style=\"font-size: 12pt;\">All payments are on invoice.<\/span><\/li>\n<\/ul>\n<p class=\"vf-box__text\">Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.<\/p>\n<\/div><\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-mediakit\"><h2>Media kit<\/h2>\n<p>Want to let others know you&#8217;re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.<\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"576\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1-576x1024.jpg\" alt=\"\" class=\"wp-image-191347\" style=\"width:177px;height:auto\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1-576x1024.jpg 576w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1-169x300.jpg 169w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1-768x1365.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1-864x1536.jpg 864w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01-MS-Igstory-1.jpg 1080w\" sizes=\"auto, (max-width: 576px) 100vw, 576px\" \/><figcaption class=\"vf-figure__caption\">Social 1080&#215;1920<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square-1024x1024.jpg\" alt=\"\" class=\"wp-image-177809\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square-1024x1024.jpg 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square-768x768.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_mediaKit_square.jpg 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">Social 1080 x 1080<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-resized\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI-1024x576.jpg\" alt=\"\" class=\"wp-image-177811\" style=\"width:264px;height:auto\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI-1024x576.jpg 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI-300x169.jpg 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI-768x432.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI-1536x864.jpg 1536w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_FB_X_LI.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">Social 1600 x 900<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide-1024x576.jpg\" alt=\"\" class=\"wp-image-177815\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide-1024x576.jpg 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide-300x169.jpg 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide-768x432.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide-1536x864.jpg 1536w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/NEB25-01_ppt_slide.jpg 1920w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">PPT Slide 16&#215;9<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"parent":0,"menu_order":0,"template":"","embl_taxonomy":[],"class_list":["post-171283","vf_event","type-vf_event","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"vf_event_start_date":{"value":"20250929","formatted":"29 September 2025"},"vf_event_end_date":{"value":"20251002","formatted":"2 October 2025"},"vf_event_submission_opening":{"value":"","formatted":""},"vf_event_submission_closing":{"value":"","formatted":""},"vf_event_registration_opening":{"value":"","formatted":""},"vf_event_registration_closing":{"value":"","formatted":""},"vf_event_summary":{"value":"","formatted":""},"vf_event_additional_info":{"value":"","formatted":""},"vf_event_registration_fee":{"value":"","formatted":""},"vf_event_canceled":{"value":"no","formatted":"no"},"vf_event_venue":{"value":"EMBL Advanced Training 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