Besides the sessions, presentations and flash talks, there were also two poster sessions during which the researchers could present their findings. Afterwards all votes were counted and five winners were announced. <\/p>\n\n\n\n
Congratulations to all of them. Now, have a look at their amazing work!<\/p>\n\n\n\n
Notch signalling dynamics in neighbouring tissues of the mouse embryo tail <\/strong><\/h2>\n\n\n\nPresenter: <\/strong>Yasmine el Azhar<\/a>, Hubrecht Institute, The Netherlands<\/p>\n\n\n\nCollaborators:<\/strong> Katharina Sonnen, Hubrecht Institute, The Netherlands<\/p>\n\n\n\n<\/p>\n\n\n\n![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/elAzhar.jpg\")
Yasmine el Azhar<\/figcaption><\/figure>\n\n\n\nAbstract:<\/strong> Biological oscillators orchestrate rhythmic patterns across diverse biological processes, which synchronize and\/or regulate temporal activities. The rhythmic patterns play a crucial role in embryonic development. Here, oscillations not only govern temporal dynamics but also spatial patterning. Thus, understanding signalling oscillations elucidates how cells differentiate, organize and form complex structures.<\/p>\n\n\n\nNotch signalling dynamics are known to regulate cell fate decisions and tissue morphogenesis in neurogenesis and somitogenesis. Both processes are present in the embryo tail, that consists of the presomitic mesoderm (PSM), the preneural tube (PNT) and the neuromesodermal progenitors (NMPs). The latter is known to give rise to both PSM and PNT cells. Even though, Notch signalling dynamics are widely studied in PSM and neural progenitors, little is known about these dynamics in the PNT and NMPs. Therefore, our study focusses on unravelling the contribution of Notch signalling dynamics to tail patterning and morphogenesis.<\/p>\n\n\n\n
To investigate Notch signalling dynamics in the embryo tail, we generated an endogenous mouse reporter. With this reporter we analysed signalling dynamics in differentiating cells of the NMP, PSM and PNT at single\u2011cell resolution. These observations were confirmed in in vitro differentiations. By perturbing signalling activity, we have analysed the influence of Notch signalling dynamics on cell fate determination. Thus, our study provides valuable insights into the role of Notch signalling dynamics in posterior vertebrate development.<\/p>\n\n\n\n
View Poster<\/a><\/p>\n\n\n\n<\/p>\n\n\n\n
<\/div>\n\n\n\nClock-driven RNA-interactome: implications and physiological significance of a post\u2011transcriptional oscillator<\/strong><\/strong><\/h2>\n\n\n\nPresenter: <\/strong>Dinesh Jadhav<\/a>, Department of Biology, Ashoka University, India<\/p>\n\n\n\nCollaborators:<\/strong> Sougata Roy, Department of Biology, Department of Biology, India <\/p>\n\n\n\n<\/p>\n\n\n\n![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Dinesh_Jadhav-002-e1712059821902-911x1024.jpg\")
Dinesh Jadhav<\/figcaption><\/figure>\n\n\n\nAbstract:<\/strong> Not available due to unpublished data<\/p>\n\n\n\nPoster: <\/strong>Not available due to unpublished data<\/p>\n\n\n\n<\/p>\n\n\n\n
<\/p>\n\n\n\n
Dinesh Jadhav is the recipient of an EMBO|EMBL Symposia fellowship, jointly funded by EMBO and the EMBL Advanced Training Centre Corporate Partnership Programme.<\/p>\n\n\n\n
<\/div>\n\n\n\nRegular fluctuations in central carbon metabolism accompany steady\u2011state growth in Escherichia coli<\/strong> <\/strong><\/h2>\n\n\n\nPresenter: <\/strong>Julian Pietsch<\/a>, Max Planck Institute for Terrestrial Microbiology, Germany <\/p>\n\n\n\nCollaborators:<\/strong> Victor Sourjik, Max Planck Institute for Terrestrial Microbiology, Center for Synthetic Microbiology, Germany <\/p>\n\n\n\n![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Julian-Pietsch-scaled-e1712061171799-950x1024.jpg\")
Julian Pietsch<\/figcaption><\/figure>\n\n\n\nAbstract:<\/strong> Bacteria usually meet the energy demands for sustained rapid growth through the catabolic breakdown of carbon precursors. It is thus generally presumed that stable growth would favour a steady flux through central carbon metabolism. <\/p>\n\n\n\nHere, we report instead that regular fluctuations in pyruvate, the end\u2011product of glycolysis, occur during normal steady\u2011state growth of Escherichia coli. Using a Fluorescence Resonance Energy Transfer (FRET) reporter for intracellular levels of pyruvate in combination with mother machine microfluidics devices, we observe fluctuations with a time scale on the order of an hour for growth of single cells on glycolytic and gluconeogenic carbon sources. Surprisingly, the amplitude rather than the frequency of fluctuations is associated with nutrient quality, variability in cell cycle length and growth. High amplitude fluctuations are observed for growth on a gluconeogenic carbon source, but they are absent in cells showing very slow or no growth and only weak in a glycolytic carbon source. <\/p>\n\n\n\n
Our results suggest that even in species optimised for fast, competitive growth, flux through central metabolism should not be considered to be held to a single fixed point, but rather as a system driven between alternative modes. <\/p>\n\n\n\n
Collaborators:<\/strong> Katharina Sonnen, Hubrecht Institute, The Netherlands<\/p>\n\n\n\n <\/p>\n\n\n\n Abstract:<\/strong> Biological oscillators orchestrate rhythmic patterns across diverse biological processes, which synchronize and\/or regulate temporal activities. The rhythmic patterns play a crucial role in embryonic development. Here, oscillations not only govern temporal dynamics but also spatial patterning. Thus, understanding signalling oscillations elucidates how cells differentiate, organize and form complex structures.<\/p>\n\n\n\n Notch signalling dynamics are known to regulate cell fate decisions and tissue morphogenesis in neurogenesis and somitogenesis. Both processes are present in the embryo tail, that consists of the presomitic mesoderm (PSM), the preneural tube (PNT) and the neuromesodermal progenitors (NMPs). The latter is known to give rise to both PSM and PNT cells. Even though, Notch signalling dynamics are widely studied in PSM and neural progenitors, little is known about these dynamics in the PNT and NMPs. Therefore, our study focusses on unravelling the contribution of Notch signalling dynamics to tail patterning and morphogenesis.<\/p>\n\n\n\n To investigate Notch signalling dynamics in the embryo tail, we generated an endogenous mouse reporter. With this reporter we analysed signalling dynamics in differentiating cells of the NMP, PSM and PNT at single\u2011cell resolution. These observations were confirmed in in vitro differentiations. By perturbing signalling activity, we have analysed the influence of Notch signalling dynamics on cell fate determination. Thus, our study provides valuable insights into the role of Notch signalling dynamics in posterior vertebrate development.<\/p>\n\n\n\n View Poster<\/a><\/p>\n\n\n\n <\/p>\n\n\n\n Presenter: <\/strong>Dinesh Jadhav<\/a>, Department of Biology, Ashoka University, India<\/p>\n\n\n\n Collaborators:<\/strong> Sougata Roy, Department of Biology, Department of Biology, India <\/p>\n\n\n\n <\/p>\n\n\n\n Abstract:<\/strong> Not available due to unpublished data<\/p>\n\n\n\n Poster: <\/strong>Not available due to unpublished data<\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n Dinesh Jadhav is the recipient of an EMBO|EMBL Symposia fellowship, jointly funded by EMBO and the EMBL Advanced Training Centre Corporate Partnership Programme.<\/p>\n\n\n\n Presenter: <\/strong>Julian Pietsch<\/a>, Max Planck Institute for Terrestrial Microbiology, Germany <\/p>\n\n\n\n Collaborators:<\/strong> Victor Sourjik, Max Planck Institute for Terrestrial Microbiology, Center for Synthetic Microbiology, Germany <\/p>\n\n\n\n Abstract:<\/strong> Bacteria usually meet the energy demands for sustained rapid growth through the catabolic breakdown of carbon precursors. It is thus generally presumed that stable growth would favour a steady flux through central carbon metabolism. <\/p>\n\n\n\n Here, we report instead that regular fluctuations in pyruvate, the end\u2011product of glycolysis, occur during normal steady\u2011state growth of Escherichia coli. Using a Fluorescence Resonance Energy Transfer (FRET) reporter for intracellular levels of pyruvate in combination with mother machine microfluidics devices, we observe fluctuations with a time scale on the order of an hour for growth of single cells on glycolytic and gluconeogenic carbon sources. Surprisingly, the amplitude rather than the frequency of fluctuations is associated with nutrient quality, variability in cell cycle length and growth. High amplitude fluctuations are observed for growth on a gluconeogenic carbon source, but they are absent in cells showing very slow or no growth and only weak in a glycolytic carbon source. <\/p>\n\n\n\n Our results suggest that even in species optimised for fast, competitive growth, flux through central metabolism should not be considered to be held to a single fixed point, but rather as a system driven between alternative modes. <\/p>\n\n\n\nClock-driven RNA-interactome: implications and physiological significance of a post\u2011transcriptional oscillator<\/strong><\/strong><\/h2>\n\n\n\n
Regular fluctuations in central carbon metabolism accompany steady\u2011state growth in Escherichia coli<\/strong> <\/strong><\/h2>\n\n\n\n
![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Elena-Tonin-1.jpg\")
![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/EES24-02_VR-copy.jpg\")
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