Presenter: <\/strong>Viktoras Rago\u017eius<\/a>, Vilnius University, Lithuania<\/p>\n\n\n\n Collaborators:<\/strong> Inga Songailiene, Tomas Sinkunas, Algirdas Miksys, Virginijus Siksnys, Lina Malinauskaite, Vilnius University, Lithuania<\/p>\n\n\n\n <\/p>\n\n\n\n Abstract:<\/strong> Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide an adaptive immunity against exogenous nucleic acids (NA) in bacteria and archaea. CRISPR-Cas systems retain a record of past infections by incorporating short segments of foreign NA as spacers within the host CRISPR array in a process known as adaptation.<\/p>\n\n\n\n Adaptation stage requires a multistep process, starting with obtaining a pool of possible DNA substrates for the adaptation complex and completing with integration of prespacers into CRISPR locus in a correct orientation to enable biogenesis of functional crRNA molecules, that are vital for the RNA-guided interference pathways.<\/p>\n\n\n\n Heterohexameric complex composed of Cas1 and Cas2 proteins (adaptation complex) identifies a potential prespacer by recognizing a specific 2-5 base pairs sequence known as protospacer adjacent motif (PAM). Within type I systems, PAM recognition can be achieved either directly through the adaptation complex or with the involvement of Cas4<\/p>\n\n\n\n (PAM-cleaving endonuclease). The coordination of PAM processing with stepwise integration is important to establish unidirectionality of prespacer\u2019s insertion into CRISPR array.<\/p>\n\n\n\n Nonetheless, this mechanism has been more extensively investigated in systems that include Cas4, as opposed to systems where adaptation complex directly recognizes the PAM. Here, single particle transmission electron cryo-microscopy was used to determinestructures of type I adaptation complex interaction with different prespacers. <\/p>\n\n\n\n This study provides important insights into the molecular basis of adaptation stage based on prespacers processing.<\/p>\n\n\n\n Poster not available due to unpublished data<\/em>.<\/p>\n\n\n\n The EMBL Industry Workshop <\/a>‘Cryo-EM in academia and industry’<\/a>\u00a0<\/a>took place from 29 \u2013 31 January 2024 at EMBL Heidelberg and virtually.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":" The first conference of the year brought together cryo-EM academic communities and industry partners to evaluate how the use of cryo-EM has developed over the last decade. The researchers provided updates on recent and ongoing methodological developments and focused on the integration of cryo-ET…<\/p>\n","protected":false},"author":72,"featured_media":1802,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[7960],"tags":[7958,7732,7956,8100],"embl_taxonomy":[],"class_list":["post-155235","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-best-poster-awards","tag-best-poster","tag-conference","tag-poster-abstract","tag-poster-prize"],"acf":[],"embl_taxonomy_terms":[],"featured_image_src":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/01\/CPP19-01.jpg","_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/posts\/155235","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/users\/72"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/comments?post=155235"}],"version-history":[{"count":2,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/posts\/155235\/revisions"}],"predecessor-version":[{"id":155741,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/posts\/155235\/revisions\/155741"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/media\/1802"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/media?parent=155235"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/categories?post=155235"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/tags?post=155235"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-json\/wp\/v2\/embl_taxonomy?post=155235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"\"](\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Viktoras-Ragozius-photo.jpg\")
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