Tuesday, October 30, 2007

FRAP analysis: Questions and Answers

For questions and comments, please feel free to post here in the comments section.

071030 ver9b
Stefan told me problem of negative Half Life value [s] for some cases, and I found out that when the data time range is too short and not reaching plateu, the calculation of HL results strange values. This is because HL in double exponential fitting is calculated by numerically interpolating the half max value.

To avoid this problem, estimation curve is not extrapolated that the HL becomes available even for the experiments with short time range. [now, still have problems to cope with "weighting" adjustments]

080325 ver9c
fixed popupmenu problem.
http://www.embl.org/cmci/weblog/2008/03/frapcalc-questions-and-answer-please.html

Labels: ,

Links to this post:

Create a Link

<< Home